Peter,

You may find that the staining corresponds to the nucleolus. I have found
that with many mRNA-ISH reactions, if there is cytoplasmic staining then
there is often nucleoloar staining as well. Not surprising since mRNA is
initially produced in the nucleus. I will upload to Histonet some pics on
this type of staining shortly.

Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

http://www.histosearch.com/homepages/TonyHenwood/default.html
http://us.geocities.com/tonyhenwoodau/index.html



-----Original Message-----
From: Peter Munday [mailto:p.munday@inpharmatica.co.uk]
Sent: Thursday, 4 December 2003 10:11 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] In-situ hybridization help please


Hello Histonetters,

I am currently doing in-situ hybridization on paraffin embedded tissue array
sections.  Very often I get a signal in the nucleus, which I think is a
little odd as I would expect to see it in the cytoplasm.  
Has anybody else encounted this?
If so, is it real?
If it isn't real, what can be done about it?

Thanks,

Peter Munday
Inpharmatica
London


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