RE: [Histonet] Cox-2 antibody from Santa Cruz

From:"Bonnie P Whitaker"

Are you getting any staining?  (Please describe exactly what the problem


-----Original Message-----
[]On Behalf Of Victoria
Sent: Wednesday, December 10, 2003 10:21 AM
To: HistoNet Server
Subject: [Histonet] Cox-2 antibody from Santa Cruz


Is anyone currently using the Cox-2 (goat
polyclonal)from Santa Cruz?  Up until my last two runs
I wasn't having any problems with it.

My concentration is 1:1000, I'm using EDTA as HIER in
a microwave (1200watt, two 3 minute cycles pl 7;
boil/simmer is approximately 30 seconds each).  I'm
using a Vector universal ABC detection kit with Nova
Red chromogen for visualization.  The tissue is human
cervical samples, and I've had good results from them
in the past.

I have replaced the antibody and the chromogen.  The
detection kit is only 3 months old.

Any input would be very much appreciated.

Vikki Baker
Institute for Cancer Prevention
Valhalla, NY  10595

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