From: | "Houston, Ronnie" |
Ronnie Houston
Regional Histology Operations Manager
Bon Secours HealthPartners=0D
Laboratories
5801 Bremo
Road
Richmond, VA 23226
(804) 287 7972
ronnie_houston@bshsi.com=0D
-----Original Message-----
From: Patsy Ruegg [mailto:pruegg@colobio.com]
Sent: Wednesday, December 03, 2003 1:19 PM
To: Jackie.O'Connor@abbott.com; Pat Flannery
Cc: Histonet@lists.utsouthwestern.edu; histonet-admin@lists.utsouthwestern.edu
Subject: RE: [Histonet]=0D Citrate Buffer pHHas anybody tried the AR reagents that deparaffinzed such as BioCare Decloaker in a steamer in place of the pressure cooker? PC is too harsh for some of my stuff but I like using these retrieval/deparaffination reagents.
Patsy
-----Original Message-----
From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Jackie.O'Connor@abbott.com
Sent: Tuesday, December 02, 2003 2:50 PM
To: Pat Flannery
Cc: Histonet@lists.utsouthwestern.edu; histonet-admin@lists.utsouthwestern.edu
Subject: Re: [Histonet] Citrate Buffer pH
I've been using a product from Biocare, a Universal Decloaker (6.0 citrate buffer) , which=0D deparaffinizes during HIER in their pressure cooker (Decloaking Chamber) - they are a very Trekkie oriented company. It works great - saves a bunch o' steps. You might try contacting them for the specifics. I don't think about the nature of these things if they work - I just follow along=0D blindly . . . . . . . . .sometimes.
Jacqueline M.=0D O'Connor HT(ASCP)
Abbott Laboratories
Global Pharmaceutical Research and Development
Discovery Chemotheraputics
Pat Flannery <pjfnefro@duke.edu>
Sent by: histonet-admin@lists.utsouthwestern.edu12/02/2003 03:00 PM
To: Histonet@lists.utsouthwestern.edu
cc:
Subject: [Histonet]=0D Citrate Buffer pH
I have a procedure for deparaffinizing (their word, not mine) tissue
sections in order to do immunostaining using the Vector MOM kit. It
calls for heating the section (briefly) in a microwave in 0.1M Citrate
Buffer.=0D The procedure does not specify the pH of the buffer, just to=0D
make it by mixing 0.1M NaCitrate and 0.1M citric acid. Does this sound
familiar to anyone out there?
--
-Patrick J. (Pat) Flannery
Division of Nephrology (that's kidneys to you)
Box 3014 (that's NOT "PO" just "Box")
Duke University Medical Center
Durham, NC 27710
E-mail: pjfnefro@duke.edu (preferred)
FLANN002@MC.DUKE.EDU (also works)
Voice: (919)660-6863
Fax: (919)684-2929
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