RE: [Histonet] Citrate Buffer pH

From:"Houston, Ronnie"

We routinely use Reveal and Borg, both from Biocare Medical, with great success in  a B&D steamer, 20 minutes, followed by 2 rinses in hot buffer, and 20 minute cool down. Very rarely use pressure cooker, and don't use micrwave at all.

Ronnie Houston
Regional Histology Operations Manager
Bon Secours HealthPartners=0D Laboratories
5801 Bremo Road
Richmond, VA 23226
(804) 287 7972

-----Original Message-----
From: Patsy Ruegg []
Sent: Wednesday, December 03, 2003 1:19 PM
To: Jackie.O'; Pat Flannery
Subject: RE: [Histonet]=0D Citrate Buffer pH

Has anybody tried the AR reagents that deparaffinzed such as BioCare Decloaker in a steamer in place of the pressure cooker?  PC is too harsh for some of my stuff but I like using these retrieval/deparaffination reagents.



-----Original Message-----
From: []On Behalf Of Jackie.O'
Sent: Tuesday, December 02, 2003 2:50 PM
To: Pat Flannery
Subject: Re: [Histonet] Citrate Buffer pH


I've been using a product from Biocare, a Universal Decloaker (6.0 citrate buffer) , which=0D deparaffinizes during HIER in their pressure cooker (Decloaking Chamber) - they are a very Trekkie oriented company.  It works great - saves a bunch o' steps.  You might try contacting them for the specifics.  I don't think about the nature of these things if they work - I just follow along=0D blindly . . . . . . . . .sometimes.

Jacqueline M.=0D O'Connor HT(ASCP)
Abbott Laboratories
Global Pharmaceutical Research and Development
Discovery Chemotheraputics


Pat Flannery <>
Sent by:

12/02/2003 03:00 PM

        Subject:        [Histonet]=0D Citrate Buffer pH

I have a procedure for deparaffinizing (their word, not mine) tissue
sections in order to do immunostaining using the Vector MOM kit.  It
calls for heating the section (briefly) in a microwave in 0.1M Citrate
Buffer.=0D  The procedure  does not specify the pH of the buffer, just to=0D
make it by mixing 0.1M NaCitrate and 0.1M citric acid.  Does this sound
familiar to anyone out there?


-Patrick J. (Pat) Flannery
Division of Nephrology (that's kidneys to you)
Box 3014 (that's NOT "PO" just "Box")
Duke University Medical Center
Durham, NC  27710
E-mail: (preferred)
        FLANN002@MC.DUKE.EDU (also works)
Voice:   (919)660-6863
Fax:     (919)684-2929

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