RE: [Histonet] Bone Marrow Biopsy


Hi Amy!  We encountered this problem many years ago.  At the time we were using RDO to decalcify our bone marrows, and we found, the hard way, that if you leave them in the acid too long they "loose" their charge.  The first thing to do is check your decal times; secondly, if you want to restore some of the charge you can immerse your specimens in a strong base.  We tried it and it helped some, but nothing substitutes good time keeping and using fresh solutions.  Good luck!
Juan C. Gutierrez, HT(ASCP)

	-----Original Message----- 
	From: Amy Self [] 
	Sent: Fri 12/5/2003 10:38 AM 
	To: '' 
	Subject: [Histonet] Bone Marrow Biopsy

	        Hello Histonetters !     Hope that everyone had a great
	         I was wandering how all of you netters fixed and decaled your bone
	marrow biopsy specimens?  Here lately the doc that reads out our BM cases
	says that the biopsy looks kind of smudgy gray.   What could this be?  He
	wants to believe that it is not getting deparaffinized enough but all of the
	other slides in the rack are just fine..... I am open for any
	Thanks in advance
	        Amy Self
	        Georgetown Memorial Hospital
	        Georgetown,  SC  29440
	        843-527-7179 (work)
	        843-520-7882 (fax)
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