RE: [Histonet] BioCare Decloaker

From:"Favara, Cynthia (NIH/NIAID)"

BioCare Decloaker
I have used this routinely for a little over a year. I do agree some of the nuclear detail is less than wonderful but since we also have an H&E usually cut serially it is not so problematic. I think the temperature range is not a tight as I would like but I have found it to be very helpful. I usually use citrate @pH 6 122C 20 minutes. Have used various buffers and even dilute HCl and not had a problem with tissue destruction or sections falling off slides.

Cynthia Favara
903 South 4th Street
Hamilton, MT 59840

-----Original Message-----
From: STURROCK, Sue []
Sent: Wednesday, December 10, 2003 2:45 PM
To: ''
Subject: [Histonet] BioCare Decloaker

Hi Guys,

We have recently purchased the BioCare Decloaker and have been impressed with our improved antigen retrieval results.  Especially for antigens such as Thyroid Transcription Factor 1 and Cytokeratin cocktail 5 & 6.  However,  it has given us some problems with spiking of high temperatures and subsequent destruction of tissue sections.  Also nuclear detail seems to be reduced when using this instrument.

Has anyone else out there had problems like this and can we expect anything else?

Sue Sturrock
Austin Health
Melbourne, Australia


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