[Histonet] pretreatment for IHC

From:"Scholz, Stephen J."

pretreatment for IHC

Hello Histonet,

I am having a problem with background staining on IHC slides.  This is only happening in cell block specimens.  Is this common on cell blocks?  Should I do a pretreatment on these?  What pretreatments do others prefer?  Should I peroxide quench?  Before or after?  What about blockers?  Do I ramble on and ask to many questions?

Thanks for all your help,

Stephen J. Scholz HT(ASCP)
Histology Coordinator
OSF St. Anthony Medical Center
Rockford IL

Phone: 815-395-5410
Fax: 815-395-5364
e-mail: sjscholz@osfhealthcare.org

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