[Histonet] polymerization problems
Dear Histonetters:
I am going to try again, realizing that not many
have vast experience with embedding large specimen with technovit 9100
yet. Maybe someone may be able to share some pointers for a non-profit
lab?
Essentially, we fail to successfully polymerize med
fem ovine condyles in approx 100ml jars. Tried at -15C with no change in
solution consistency for a week; then brought it up to -2C still no change over
three days. Jars were "vacuumed" and set in refrigerator / freezer.
I am at the of my rope of troubleshooting.
Thanks for any pointers.
Tom
------------------------------------------
Thomas P. Schaer, VMD
Assist. Prof. BIOMED, Drexel University
Comparative
Orthopaedic Research & Tissue Engineering
Department of Clinical
Studies
University of Pennsylvania New Bolton Center
382 West Street
Road
Kennett Square, PA 19348
tel. 610-444-5800 (x6261 office)
tel.
610-444-5800 (x6131 lab)
fax. 610-925-8100
tpschaer@mail.vet.upenn.edu
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