[Histonet] mouse embryo section tears

From:Rashmi Chandra

I am sectioning paraffin embedded mouse embryos (fixed in 4% 
paraformaldehyde) at 7 microns. The sections cut and ribbon fine but after 
i dry them on the slide, tears appear in the liver. These tears are not 
present when I first check the sections after lifting them from the water 
bath (temperature 37 C) onto the slide.  I think the tears may result from 
differential drying of the various tissues, and I am looking for a way to 
prevent them.

Thanks a lot for your advice!

Rashmi Chandra, Ph.D.
Dept. of Neurobiology
Duke University Medical Center
Durham, NC 27710

Tel: (919) 681-5449 

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