[Histonet] The best staining method for new bone formation

From:Ronakorn Panjaphongse

Hello everybody
I would like to conduct the study to evaluate the action of Bone Morphogenetic Protein (BMP) antagonist in prevent new bone formation. But the well known H&E stain may be not good enough because both matrix and new bone formation ( osteoid ) is also red, and it is also very hard in quantitative evaluation by using computer program ,eg. NIH too. 
Does anybody has some advice in better staining method or other way in quantitative evaluation that able to differentiate the new bone formation from the peripheral matrix.
Thank you very much for your answer in advance.
Ronakorn Panjaphongse
Graduate Student
Graduate School of Biomedical Science
Hiroshima University

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