[Histonet] Re: Stain for H.Pylori


Lee and Peggy Wenk offer a Diff-Quik technique for Helicobacter pylori.

My personal preference - supported by at least some of the literature - is to omit the Diff-Quik I (eosin). - I've seen most of the stains in current use, in the various labs I work in. I think that IHC makes it easier to work without oil immersion. My current practice with Diff-Quik II is to examine all positives and many of the apparent negatives with a 100x oil immersion lens. I think this really makes a difference, particularly if your high-dry (45x) objective is less than perfect.

Diff-Quik is of course a trade name. My personal experience is that generics work quite as well.

Bob Richmond
Samurai Pathologist
Knoxville TN

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