[Histonet] Perfused versus non-perfused tissue for IHC
Dear All
For IHC some researchers use perfused tissue section. Others use
non-perfused tissue for the study of the same antigen. My questions are:
1. What are the advantages of using perfused tissue over non-perfused tissue
for IHC staining?
2. For what type of antigen study tissue perfusion is essential?
3. What type of differences may I encounter between perfused and
non-perfused tissue stained for the same antigen?
Thank you in advance for answer and clarification.
Please let me know any link (like website or article) where I can get detail
discussion on this issue
Subrata Biswas
Nephrology Div of Internal Med
UNICAMP, SP, Brazil.
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