Does anyone know why you're supposed to make up 0.2M PBS for 
perfusion or fixation of slides (to make up neutral buffered formalin 
or paraformaldehyde) fresh?  I understand why it's important to make 
up the formalin or paraformaldehyde fresh, but what could happen to 
the PBS (unless something grows in it).  Is it really important to 
make it up fresh?

Sharon
-- 
Sharon Cooperman        	     
NIH, NICHD, CBMB                     301.435-7735
Building 18T, room 101               301.402-0078 fax
Bethesda, MD 20892

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