[Histonet] Make of Toluidine blue solution

From:"Lina Vieira"

Helo Everyone,
In our lab usually, we make the toluidine solution (for coloration of resin included tissue):
1g - Borax
1g  - Toluidine Blue
100 - ml water,
1 minute - staining time
And because, now, I have another protocol for make the solution, I want ask you about the possibility to use this solution, and if is it necessary change the staining time?
 New protocol:
0.2 g - Toluidine Blue
0.5 - Acetic acid
Complete, with desionized water, to 100 ml
Tanks in advance
Lina Vieira

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