I am using a protocol from Ruzin's Plant Microtechnique book. I'm not sure 
if this technique is common but it does involve picric acid. I believe
that the 
picric acid stops the safranin from overstaining.

A. Bishop
San Francisco State University

On Thu, 19 Dec 2002 12:35:27 -0500 "J. A. Kiernan" wrote:

> I've looked in a few books and cannot find a variant
> of Johansen's safranine-light green (or -fast green FCF)
> that involves picric acid. Is your technique something
> other than the commonly used stain for sections of
> plant tissue?  (The same combination of dyes has been
> used for cytoplasmic organelles in sections of 
> osmium-fixed animal material, but I don't think it
> has a big following for that purpose these days.)
> -- 
> -------------------------
> John A. Kiernan
> Department of Anatomy and Cell Biology
> The University of Western Ontario
> London,   Canada   N6A 5C1
>    kiernan@uwo.ca
>    http://publish.uwo.ca/~jkiernan/
> -----------------------------------

-------
> abishop@sfsu.edu wrote:
> > 
> > Has anyone out there ever used HCl as a substitute for Picric acid in a
> > staining protocol such as Johansen's Safranin/Fast Green? If so
> what were
> > the results? Thanks for reading this.
> > 
> > A. Bishop
> > San Francisco State University
> > 1600 Holloway Ave
> > San Franicso, CA 94132
> 



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