Seeking H&E stains procedure for Ground Plastic section
From: | Bernard Ian R SSgt 59 CRES/MSROP |
Hello again, to all fellow,
Histo Plastic Researchers,
My Pathologist is adamant in me, finding a H&E procedure
that will work, to stain some plastic processed (Methyl Methacrylate) pig
& rabbit eye implants. I mentioned to him about other stains out
there, that work similarly to the H&E and stains
better. However, he insists that the H&E will give him a
familiar and better contrast.
The one method I have, worked
(acceptable to him) for another plastic procedure( A Butyl Methyl
Methacrylate), but not this one. The stain came out to light. I
even restained the slides following the same procedure again. And it still
turned out light.
My adopted Plastic
H&E Method abbreviated to key reagents:
1. I surface etched the plastic
slides before staining to allow for stain penetration in 1 % Formic Acid ( 2
mins), 50/50 ETOH and rinse in Distilled H20.
2. Stain 6 min in Harris
Hematoxylin
3. Differentiate in 0.5 % Acid
Alcohol ( HCL in 70 % Alcohol)
4. Blue in Ammonia water
(12 drops Ammonium Hydroxide in 250 mls Distilled
water)
5. Stain 1 min in Eosin Y
solution
6. Dehydrate to Xylene
substitute.
These sections are less than
100 microns thick, no less than 40 microns
I need help with a good
procedure.
Thanks in
advance
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