Re:variability on staining

From:Carlos Defeo

 Dear Ali:
Can you tell the formula of the eosin solution you are using? For the
changes in ethanol 95 I suppose you are employing an alcoholic solution.
I recommend an aquous solution of eosin Y 0,75-1%
and ommit then the wash in water,passing trough graded alcoholic baths. On
this way you will have to change the first 95 ethanol dehydrating bath
frequently but overdifferenciation of eosin will be avoided.

<< Previous Message | Next Message >>