Re: pepsin

From:"John C. Dennis"


Start with the cheapist.  Sigma P-7000 is what I bought only last week.
If you don't have a protocol for the number of units/unit vol., ball park
some number 10x more than what you want.  Make stocks in PBS then dilute
1:10 with 0.1 N HCl.

John Carroll Dennis
Anatomy, Physiology, and Pharmacology
109 Greene Hall
Auburn University, AL  36849

On Mon, 16 Dec 2002, Elizabeth Chlipala wrote:

> Hello All
> I need to use pepsin for epitope retreival.  I checked in Sigma to order and
> they have about 5 different versions.  All have different units/mg of
> protein.  Does anyone have a suggestion on where I should start.
> Thanks in advance
> Liz Chlipala

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