Start with the cheapist. Sigma P-7000 is what I bought only last week.
If you don't have a protocol for the number of units/unit vol., ball park
some number 10x more than what you want. Make stocks in PBS then dilute
1:10 with 0.1 N HCl.
John Carroll Dennis
Anatomy, Physiology, and Pharmacology
109 Greene Hall
Auburn University, AL 36849
On Mon, 16 Dec 2002, Elizabeth Chlipala wrote:
> Hello All
> I need to use pepsin for epitope retreival. I checked in Sigma to order and
> they have about 5 different versions. All have different units/mg of
> protein. Does anyone have a suggestion on where I should start.
> Thanks in advance
> Liz Chlipala
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