Are you asking how to stain all mast cells?
If so, the answer is that you must use a cationic
dye, not an antibody. 
-- 
-------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan@uwo.ca
   http://publish.uwo.ca/~jkiernan/
_______________________________________________
alireza a wrote:
> 
> Hi dear colleagues
> 
> I am trying to study the mast cells in the vessels walls of
> mouse.I would appreciate if any one expert in this field to
> help me know:
> 
> 1-Which method is most sensitive in immunohistochemical
> immaging of mast cells in the adventitia of mouse vessels.I am
> trying to use monoclonal anti MMCP1/2 or MMCP6.Is that a good
> choice.
> 
> 2-Where can I obtain specific anti mouse mast cell protease
> monoclonal antibody? I would appreciate to be informed of any
> supplier.