From:Katri Tuomala

I don't seem to be able to get all the basal cells in prostate staining with
protease. I use tonsil/prostate combination as a control. Tonsil stains
fine, but a lot of basal cells in prostate do not stain, whereas with HIER I
get good staining. Which protease do you use and what detection system?
Maybe my detection is not sensitive enough.


----- Original Message -----
To: ; ;

Sent: Monday, December 16, 2002 9:17 AM
Subject: Re: IHC

> I have found in my experience that sometimes steam retrieval methods can
"open"too many nuclei to staining , I have even forced false positivity by
steaming too long, it is a very touchy balance. however on 34be12 we use
enzyme not steam, with no problems and do the same on cytokeratins. we use a
protease for anywhere from 2 to 6 minutes and have no problems. hope this
helps. dana

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