I have found in my experience that sometimes steam retrieval methods can "open"too many nuclei to staining , I have even forced false positivity by steaming too long, it is a very touchy balance. however on 34be12 we use enzyme not steam, with no problems and do the same on cytokeratins. we use a protease for anywhere from 2 to 6 minutes and have no problems. hope this helps. dana

<< | Next Message >>