Dear Ian,

Is it possible that your colleague is confusing APES with some of the other, 
older section adhesives, most notably those containing albumin? These were 
notorious for creating background problems.

Louise Renton
Bone Research Unit
MRC
Johannesburg
South Africa
Tel & fax +27 11 717 2298
"Time flies like an arrow, fruit flies like a banana"





>From: "clarke.ian" 
>To: histonet 
>Subject: APES slides and special stains
>Date: Tue, 17 Dec 2002 19:12:02 +0000
>
>Dear all,
>Could someone tell me if there are any problems with using APES coated
>slides in special stains.A colleague of mine has told some of the trainees
>that they where not to put sections onto Apes coated slides as this would
>lead to contamination and background staining,particularly when doing a PAS
>and some of the silver stains.This lead to an intense discussion and I 
>would
>like to know if this is the case.It was pointed out that we have used these
>for nail beds and bone trephines and I havn't noticed any background
>staining from the special stains.
>If this is the case and APES is PAS +VE and there is background staining of
>the APES slides could you e-mail references.
>
>Ian Clarke
>Histopath/Cytopath
>Craigavon Area Hospital


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