Re: APES slides and special stains

From:Geoff McAuliffe

    The best way to answer this question is for you to run the special stains
you use on the APES slides you make or purchase and then you will really know.
Some other lab may have different results due to all sorts of variables.
    "The proof of the pudding is in the eating"

"clarke.ian" wrote:

> Dear all,
> Could someone tell me if there are any problems with using APES coated
> slides in special stains.A colleague of mine has told some of the trainees
> that they where not to put sections onto Apes coated slides as this would
> lead to contamination and background staining,particularly when doing a PAS
> and some of the silver stains.This lead to an intense discussion and I would
> like to know if this is the case.It was pointed out that we have used these
> for nail beds and bone trephines and I havn't noticed any background
> staining from the special stains.
> If this is the case and APES is PAS +VE and there is background staining of
> the APES slides could you e-mail references.
> Ian Clarke
> Histopath/Cytopath
> Craigavon Area Hospital

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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