|From:||Kid'surgeon * |
thanks for reply.
i am staining one antibody at one time.
DMSO is to wash out the zamboni and enhance penetration.
i use 1% triton-X, what lower percentage do u suggest me to use?
I am using whole mount tech as i am looking for the enteric nervous sytem. It gives a paramount view of the gur musculature and one can nicely c the whole nerve plexues beautifully, which i guess is very much impossible to get in sections.....my view
i have not tried other fixatives....may be need to switch from zamboni to these at some stage...
have someone in the group used counterstains for this this problem of autofluorescence? if yes, what and can he/she send me the ref for methods etc