RE: Veterinary antibodies...
Dear Jim, We do some work for a Vet lab and have used S100, SMA, CD3 from Dako on dogs with good results. We think CD79a works as well but have had only 1 positive, (we don't have a positive canine control). CD3 and CD79a from Cell Marque have also been used. David.
Here are a couple of saved emails that might be useful,
Although we are doing our IHC mostly on human tissue, we occasionally help
out our colleagues in vet path. We found clone MNF116 (available from
various vendors, e.g. Dako M0821) to work very nicely in dogs. MNF116 is a
'broad-spectrum' anti-CK mAb that recognizes CKs 5, 6, 8, 17 and possibly
19. CKs 5 and 17 are basic CKs of squamous, CK 8 is typical for non-squamous
epithelia. Alternatively you can try Dako's polyclonal rb-a-Keratin, which
is another broad-spectrum Ab reactive in dogs. Good luck!
Best regards
Andi Kappeler
Institute of Pathology, University of Bern, Switzerland
Factor VIII (von Willebrand Factor) - DAKO; Cat. # A008202; Proteinase K
> enzyme pretx - 5 min. room temp.; Ab - 1:400 dil. - 30 min. rt
>
> Keratin, Pan; clone Lu-5 - NeoMarkers; Cat. # MS-744-A; Proteinase K
> enzyme
> pretx - 5 min. rt; Ab - 1:100 dil. - 30 min. rt (best one for
> hepatocytes;
> strongest staining results)
>
> Cytokeratin, broad spectrum; clone MNF116 - DAKO; Cat. # M082101;
> Proteinase
> K enzyme pretx - 5 min. rt; Ab - 1:600 dil. - 30 min. rt
>
> Cytokeratin, high mol. wt.; clone 34BE12 - DAKO; Cat/ # M063001;
> Proteinase
> K enzyme pretx - 5 min. rt; Ab - 1:50 dil. - 30 min. rt (squamous, some
> ductal epithelium)
>
> Cytokeratin, low mol. wt.; clone NCL5D3 - ICN; Cat. # 69-178-1;
> Proteinase K
> enzyme pretx - 5 min. rt; Ab - 1:10 dil. made up fresh before each use -
> 30
> min. rt (simple, glandular epithelium)
>
> LSAB2 kit used for link and label - 15 min. incubations for each
> reagent;
> AEC chromogen - 10 min. incubation
>
> If you have any other inquiries about antibodies used on canine tissue,
> I
> might be able to help with those, too. I employ approximately 60
> antibodies
> in my lab that cross-react with canine, feline, etc.
>
> Jan Shivers
> Univ. of Minnesota
> Dept. of Veterinary Diagnostic Medicine
> Immunohistochemistry Lab
> 612-625-1732; 612-624-7297
David Taylor
Laboratory Manager
Drs King & Mower
Adelaide, Australia
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