RE: Fixing a Frozen Section Slide.

From:Tony Henwood

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Depends on what you are staining for eg: 
For H&E, Perl's PAS ... I would recommend Ethanol 
Gomori's Trichrome for Muscle biopsies - air dried 
For IPs either air dried or ethanol depending on the antibody 
Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC) 
Laboratory Manager 
The Children's Hospital at  Westmead, 
Locked Bag 4001, Westmead, 2145, AUSTRALIA. 
Tel: (02) 9845 3306 
Fax: (02) 9845 3318 
http://www.histosearch.com/homepages/TonyHenwood/default.html 
http://us.geocities.com/tonyhenwoodau/index.html 


-----Original Message----- 
From: Banjo Adesuyi [mailto:badesuyi@hotmail.com] 
Sent: Monday, 16 December 2002 3:57 
To: histonet@pathology.swmed.edu 
Cc: badesuyi@hotmail.com 
Subject: Fixing a Frozen Section Slide. 


Hi, 
   Please I am wondering If you guys could advice me on the best way to fix
a frozen section slide before staining. 
   You people are doing a wonderful job. You are the best. 
     
   Ade. 



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RE: Fixing a Frozen Section Slide.



Depends on what you are staining for eg:
For H&E, Perl's PAS ... I would recommend Ethanol
Gomori's Trichrome for Muscle biopsies - air dried
For IPs either air dried or ethanol depending on the antibody
Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318
http://www.histosearch.com/homepages/TonyHenwood/default.html
http://us.geocities.com/tonyhenwoodau/index.html


-----Original Message-----
From: Banjo Adesuyi [mailto:badesuyi@hotmail.com]
Sent: Monday, 16 December 2002 3:57
To: histonet@pathology.swmed.edu
Cc: badesuyi@hotmail.com
Subject: Fixing a Frozen Section Slide.


Hi,
   Please I am wondering If you guys could advice me on the best way to fix a frozen section slide before staining.
   You people are doing a wonderful job. You are the best.
    
   Ade.



Add photos to your e-mail with MSN 8. Get 2 months FREE*.


**********************************************************************
This email and any files transmitted with it are confidential and
intended solely for the use of the individual or entity to whom they
are addressed. If you are not the intended recipient, please
delete it and notify the sender.

Views expressed in this message and any attachments are those
of the individual sender, and are not necessarily the views of the
Childrens Hospital at Westmead

This footnote also confirms that this email message has been
virus scanned and although no computer viruses were detected,
the Childrens Hospital at Westmead accepts no liability for any
consequential damage resulting from email containing computer
viruses.
**********************************************************************

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