Kim,

Did you try applying the antibody off machine.  When we used the Ventana we did indeed have two or three antibodies that we applied offline, and it made all the difference. These antibodies were evidently heat labile, and may not have been too partial to the agitation either.

We put the slides on the instrument for the detection system.

Feel free to contact me for more info.

Wanda Shotsberger HT (ASCP)

 

 

-----Original Message-----
From: Kim Saunders [mailto:cactusmoon782000@yahoo.com]
Sent: Friday, December 27, 2002 4:20 PM
To: histonet@pathology.swmed.edu
Subject: DBA44

 

Hi,

I'm trying to work up the DBA44 (Dako) on the Ventana ES IHC Stainer. I am currently trying the recommended 1:25 dilution for 32 minutes. I've tried our NordicWare pressure cooker Antigen Retrieval method (20 in the microwave, cool down for 20 minutes), and Protease 1 for 4 & 8 minutes, and Protease 2 for 8, 12, 16, & 20 minutes. I have a bone marrow bx that the pathologist is sure should be positive based on the flow results. Since my ES uses heat, could the heat be an issue? Any help would be greatly appreciated.

Thank you,

Kim Mann-Saunders

St. Joseph Hospital-Tucson, AZ

Kim Saunders
Proud to be an American!!

 

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