RE: CD138

From:"Dawson, Glen"

I have used both this Serotec antibody and the DAKO CD138 (Clone MI15).  I
had much better results with the DAKO antibody and was able to titer it out
further to reduce cost.  pH 6.0 citrate HIER @ a 1:100 dilution.
Good Luck,
Glen Dawson.

-----Original Message-----
From: Patricia Bourne []
Sent: Thursday, December 19, 2002 10:00 AM
To: Nader, Alexander
Cc: HistoNet Server
Subject: RE: CD138

We are also using the Serotec antibody ....we use Dako Citrate solution at
pH 6.1  in a Microwave pressure cooker for 40 minutes.  The articles I read
said it required very heavy retrieval.  I have not tried it with our regular
method yet which is the Rice Steamer for 30  minutes.  Our dilution with
this method is 1-200 with a staining time of 45 minutes using the Enivision
Plus system on a Dako Autostainer.  Tonsil is a good control.  


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