RE: CD138

From:Patricia Bourne

We are also using the Serotec antibody ....we use Dako Citrate solution at pH 6.1  in a Microwave pressure cooker for 40 minutes.  The articles I read said it required very heavy retrieval.  I have not tried it with our regular method yet which is the Rice Steamer for 30  minutes.  Our dilution with this method is 1-200 with a staining time of 45 minutes using the Enivision Plus system on a Dako Autostainer.  Tonsil is a good control. 



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