We are also using the Serotec antibody ....we use Dako Citrate solution at pH 6.1 in a Microwave pressure cooker for 40 minutes. The articles I read said it required very heavy retrieval. I have not tried it with our regular method yet which is the Rice Steamer for 30 minutes. Our dilution with this method is 1-200 with a staining time of 45 minutes using the Enivision Plus system on a Dako Autostainer. Tonsil is a good control.
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