RE: CD138

From:"Nader, Alexander"

>      What clone are people using for CD138 IHC?
>      

we use Sertotec's CD 138/Syndecan-1	MCA 681H, clone 
B-B4,  1:40. HIER with EDTA pH 8 in a household cooker for 10 min. (product
data sheet:
http://serotec.oxi.net/asp/datasheet.asp?code=MCA681&vers=120202).

Although Sertoc recommends HIER with citrate buffer pH 6, we prefer EDTA for
this ab.

hope that helped...

Alexander Nader MD
Vienna, Austria



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