From: | "Nader, Alexander" |
> What clone are people using for CD138 IHC? > we use Sertotec's CD 138/Syndecan-1 MCA 681H, clone B-B4, 1:40. HIER with EDTA pH 8 in a household cooker for 10 min. (product data sheet: http://serotec.oxi.net/asp/datasheet.asp?code=MCA681&vers=120202). Although Sertoc recommends HIER with citrate buffer pH 6, we prefer EDTA for this ab. hope that helped... Alexander Nader MD Vienna, Austria