RE: Analysis of sirius red with polarized light
|From:||"Marshall Terry Dr, Consultant Histopathologist" |
It is a simple fact that small amounts of amyloid will not polarise to the extent (vividness) of large amounts. The struggle then is to identify them. If they stain with Sirius red are in an appropriate place, and have the right appearance, then any amount of light to polarise it is OK with me.
In parenthesis, why are the controls for amyloid always from solid amyloid spleens etc.? That is to say - loaded.
Surely the faintest amount of amyloid is the appropriate control.
Terry L Marshall B.A.(Law), M.B.Ch.B., F.R.C.Path
Rotherham General Hospital, Yorkshire
From: firstname.lastname@example.org [mailto:email@example.com]
Sent: 17 December 2002 15:16
Subject: Analysis of sirius red with polarized light
In the process of analysis of our picro sirius red stained sections we ran
into a discussion on the microscope prefs. We could not come to a conclusion
as to how much light must be given. We use 2 polarization filters in crossed
position. Some people want to give minimal light in order to prevent
overestimation of collagen content, total background is black. Others say to
give full light, but than the background becomes somewhat blueish, which
cannot be turned to black by adjusting the filters.
Does anyone have experience with different lighting on sirius sections? Or is
anyone aware of literature in which this problem is discussed.
Thanks in advance,
Dept. of Experimental Cardiology
Thoraxcentre, Erasmus MC Rotterdam
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