Paraffin Tape Transfer System
Hi all,
I have the Instrumedics Paraffin Tape Transfer system and I haven't
had very good results with it yet. I'm wondering if anybody out
there can give me some pointers on using it and getting consistent results.
Essentially I start out with a cold (approx. 0-2 degrees C),
dry paraffin block, not iced or wet. I apply the tape and roll it
with the roller, and take one cut. Things look fine to this point.
I place the tape onto a coated slide and roll again with the
roller. (There usually appear to be places that are obviously
thicker than others on the slide, but I always roll it firmly to
try to get the best adhesion.) I then cure the slides, tape side
down, using the provided UV light fixture for 60 seconds. I take
the slides to the hood and place them in the hydrocarbon solution
also provided with the system, and slowly (very, very slowly) peel
the tape off in a diagonal manner. I have allowed the slides to sit
in the hydrocarbon for up to 10 minutes and see no difference in
performance (i.e. the tape does not come off easier).
I have yet to be able to keep all of my sections on the glass slide.
I have tried this with mouse femurs and now mouse heads. I have
tried using room temperature blocks, and this doesn't work well
either. I have tried different cutting angles of my blade, used
different blades from different vendors, and changed paraffin.
I have cured slides from 30 to 90 seconds. I have placed the tape
on the slide and then rolled it flat, and have also tried rolling
the tape onto the slide in case I have bubbles under the tape.
I'm really at a loss as to what to do next. I know some folks have
used this system for the tissue microarrays, so, somebody...help?
Egg nog (with a little less egg and a little more nog) to the folks
who can help me with this!
Thanks!
Teri Johnson
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri 64110
tjj@stowers-institute.org
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