Paraffin Tape Transfer System
I have the Instrumedics Paraffin Tape Transfer system and I haven't
had very good results with it yet. I'm wondering if anybody out
there can give me some pointers on using it and getting consistent results.
Essentially I start out with a cold (approx. 0-2 degrees C),
dry paraffin block, not iced or wet. I apply the tape and roll it
with the roller, and take one cut. Things look fine to this point.
I place the tape onto a coated slide and roll again with the
roller. (There usually appear to be places that are obviously
thicker than others on the slide, but I always roll it firmly to
try to get the best adhesion.) I then cure the slides, tape side
down, using the provided UV light fixture for 60 seconds. I take
the slides to the hood and place them in the hydrocarbon solution
also provided with the system, and slowly (very, very slowly) peel
the tape off in a diagonal manner. I have allowed the slides to sit
in the hydrocarbon for up to 10 minutes and see no difference in
performance (i.e. the tape does not come off easier).
I have yet to be able to keep all of my sections on the glass slide.
I have tried this with mouse femurs and now mouse heads. I have
tried using room temperature blocks, and this doesn't work well
either. I have tried different cutting angles of my blade, used
different blades from different vendors, and changed paraffin.
I have cured slides from 30 to 90 seconds. I have placed the tape
on the slide and then rolled it flat, and have also tried rolling
the tape onto the slide in case I have bubbles under the tape.
I'm really at a loss as to what to do next. I know some folks have
used this system for the tissue microarrays, so, somebody...help?
Egg nog (with a little less egg and a little more nog) to the folks
who can help me with this!
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri 64110
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