LN fixative

From:Carlos Defeo

 Dear Naaveda:
I think you can try with Bouinīs fixative, -remeber to do two or three
changes in ethanol 70š after fixation- but a well fixation including
preservation of antigenicity is achieved with neutral formalin 10%. In most
cases you can section the limph node in two hemisections,so you can try to
fix one in Bouin and the other in neutral formalin. Bouinīs gives  better
global preservation for a routine panel of techniques (HE,Giemsa,etc)
formalin fixation,instead, gives you better preservation of tissue antigens
for IHC studies.
Carlos,Montevideo,Uruguay



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