Factor 8
Hello, happy holidays everyone!
I am still looking for a reliable source (and protocol if possible) for staining for Factor 8 expression in RAT hepatocytes. (We are looking for cytoplasmic staining, not endothelial) If anyone could help me out with this I would greatly appreciate it!!
Thanks,
Melissa
-----Original Message-----
From: HistoNet Server [mailto:histonet@pathology.swmed.edu]
Sent: Tuesday, December 24, 2002 9:57 PM
To: HistoNet Server
Subject: Daily Digest
----------------------------------------------------------------------
Date: 24 Dec 2002 00:08:19 -0600
From: "Brothers, Julie {CNS~Palo Alto}"
Subject: Out of Office AutoReply: Daily Digest
Hello - I am currently out of the office and will be back on Monday, January
6th. I will not be checking my email.
Thanks, Julie
----------------------------------------------------------------------
Date: 24 Dec 2002 02:59:06 -0600
From: ALLISON@CARDIFF.AC.UK
Subject: Individual characteristics
Tolerance is a virtue
Russ Allison,
Dental School
Cardiff
Wales
----------------------------------------------------------------------
Date: 24 Dec 2002 07:25:50 -0600
From: Vicki Gauch
Subject: Happy Holidays
I just wanted to wish everyone a very Merry Christmas, Happy Holidays, and a
Happy, Healthy New Year !!! Thank you for all of your help over the past
year...Hope you all know how much you are appreciated !!!
Vicki Gauch
AMCH
Albany, NY
----------------------------------------------------------------------
Date: 24 Dec 2002 08:42:06 -0600
From: AliNeumann@aol.com
Subject: Variability of histology staining-request for input
Hi everyone, I am the director of a new small outpatient laboratory, and we
have had a very persistent and recalcitrant problem with variability of
staining (some sections light, others dark, especially on eosin but to a
lesser degree hematoxylin). We are soaking slides in xylene for 3 minutes in
each of 3 bins with agitation, then staining them on a Varistain 24-4 with a
regressive stain. Our staining protocols have been:
1. 100% Alcohol 20 seconds
2. 100% Alcohol 10 seconds
3. 95% Alcohol 10 seconds
4. Tap Water 10 seconds
5. Hematoxylin 7 minutes
(Protocol Harris mercury free non-acidified type)
6. Tap Water 20 seconds
7. Acid Alcohol 2 seconds
8. Tap Water 20 seconds
9. Ammonia Water 2 minutes
10. Tap Water 10 seconds
11. 95% Alcohol 10 seconds
12. Eosin 15 seconds
13. 95% Alcohol 10 seconds
14. 95% Alcohol 10 seconds
15. 100% Alcohol 20 seconds
16. 100% Alcohol 20 seconds
1. 100% Ethanol 40 seconds
2. 100% Ethanol 40 seconds
3. 95% Ethanol 40 seconds
4. Tap water 60 seconds
5. Hematoxylin 10 minutes
( Protocol Harris mercury free non-acidified type)
6. Tap water (sink) 60 seconds
7. Acid Alcohol 2 seconds
8. Tap water (sink) 60 seconds
9. Ammonia water 2.5 minutes
10. Tap water 5 minutes
11. 95% EtOH 1 minute
12. Eosin 10 seconds
13. 95% EtOH 10 seconds
14. 95% EtOH 10 seconds
15. 100% EtOH 40 seconds
16. 100% EtOH 40 seconds
We have had the same problem with both protocols, and process mostly small
skin biopsies.
Our acid and ammonia solutions are as follows:
1% acid alcohol
2000 ml of 70% EtOH
5 ml of HCl (hydrochloric acid)
1% Ammonia water
1980 cc tap water
40 ml of ammonium hydroxide
We have tried longer xylene pre-treatment with more agitation, and wiping off
alcohol drops before removing the bin from the last alcohol, as well as
various staining adjustments. We process most of our tissue on a short 3
hour cycle however believe this problem has persisted even on
overnight-processed tissue.
I would very much appreciate any advise any of you can give me on this
problem. Thanks very much and have a happy holiday! Alice Neumann M.D.
Precision Pathology PC, Arvada CO alineumann@aol.com 303-432-7855
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Content-transfer-encoding: 7BIT
Hi everyone, I am the director of a new small outpatient laboratory,
and we have had a very persistent and recalcitrant problem with variability of
staining (some sections light, others dark, especially on eosin but to a
lesser degree hematoxylin). We are soaking slides in xylene for 3
minutes in each of 3 bins with agitation, then staining them on a Varistain
24-4 with a regressive stain. Our staining protocols have been:
1. 100% Alcohol 20 seconds
2. 100% Alcohol 10 seconds
3. 95% Alcohol 10 seconds
4. Tap Water 10 seconds
5. Hematoxylin 7 minutes
(Protocol Harris mercury free non-acidified
type)
6. Tap Water 20 seconds
7. Acid Alcohol 2 seconds
8. Tap Water 20 seconds
9. Ammonia Water 2 minutes
10. Tap Water 10 seconds
11. 95% Alcohol 10 seconds
12. Eosin
15
seconds
13. 95% Alcohol 10 seconds
14. 95% Alcohol 10 seconds
15. 100% Alcohol 20 seconds
16. 100% Alcohol 20 seconds
1. 100% Ethanol 40 seconds
2. 100% Ethanol 40 seconds
3. 95% Ethanol 40 seconds
4. Tap water
60 seconds
5. Hematoxylin 10 minutes
( Protocol Harris mercury free
non-acidified type)
6. Tap water (sink) 60 seconds
7. Acid Alcohol 2 seconds
8. Tap water (sink) 60 seconds
9. Ammonia water 2.5 minutes
10. Tap water 5 minutes
11. 95% EtOH 1 minute
12. Eosin 10 seconds
13. 95% EtOH 10 seconds
14. 95% EtOH 10 seconds
15. 100% EtOH 40 seconds
16. 100% EtOH 40 seconds
We have had the same problem with both protocols, and process mostly small
skin biopsies.
Our acid and ammonia solutions are as follows:
1% acid alcohol
2000 ml of 70% EtOH
5 ml of HCl (hydrochloric acid)
1% Ammonia water
1980 cc tap water
40 ml of ammonium hydroxide
We have tried longer xylene pre-treatment with more agitation, and wiping off
alcohol drops before removing the bin from the last alcohol, as well as
various staining adjustments. We process most of our tissue on a short 3
hour cycle however believe this problem has persisted even on
overnight-processed tissue.
I would very much appreciate any advise any of you can give me on this
problem. Thanks very much and have a happy holiday! Alice
Neumann M.D. Precision Pathology PC, Arvada CO alineumann@aol.com
303-432-7855
- --Boundary_(ID_bhUjtUw8uDbvuUrCeMTk9Q)--
----------------------------------------------------------------------
Date: 24 Dec 2002 08:56:25 -0600
From: Kim Merriam
Subject: Re: Variability of histology staining-request for input
I would try longer rinses in water after the hematoxylin,
acid alcohol and ammonia water (running water would probably
be best). Also I would go longer in the 95% alcohols that
are after the eosin.
Kim Merriam
TKT
Cambridge, MA
AliNeumann@aol.com wrote:
> Hi everyone, I am the director of a new small outpatient
> laboratory, and we have had a very persistent and
> recalcitrant problem with variability of staining (some
> sections light, others dark, especially on eosin but to a
> lesser degree hematoxylin). We are soaking slides in
> xylene for 3 minutes in each of 3 bins with agitation,
> then staining them on a Varistain 24-4 with a regressive
> stain. Our staining protocols have been:
>
> 1. 100% Alcohol 20 seconds
> 2. 100% Alcohol 10 seconds
> 3. 95% Alcohol 10 seconds
> 4. Tap Water 10 seconds
> 5. Hematoxylin 7 minutes
> (Protocol Harris mercury free non-acidified type)
> 6. Tap Water 20 seconds
> 7. Acid Alcohol 2 seconds
> 8. Tap Water 20 seconds
> 9. Ammonia Water 2 minutes
> 10. Tap Water 10 seconds
> 11. 95% Alcohol 10 seconds
> 12. Eosin 15 seconds
> 13. 95% Alcohol 10 seconds
> 14. 95% Alcohol 10 seconds
> 15. 100% Alcohol 20 seconds
> 16. 100% Alcohol 20 seconds
>
>
> 1. 100% Ethanol 40 seconds
> 2. 100% Ethanol 40 seconds
> 3. 95% Ethanol 40 seconds
> 4. Tap water 60 seconds
> 5. Hematoxylin 10 minutes
> ( Protocol Harris mercury free non-acidified type)
> 6. Tap water (sink) 60 seconds
> 7. Acid Alcohol 2 seconds
> 8. Tap water (sink) 60 seconds
> 9. Ammonia water 2.5 minutes
> 10. Tap water 5 minutes
> 11. 95% EtOH 1 minute
> 12. Eosin 10 seconds
> 13. 95% EtOH 10 seconds
> 14. 95% EtOH 10 seconds
> 15. 100% EtOH 40 seconds
> 16. 100% EtOH 40 seconds
>
> We have had the same problem with both protocols, and
> process mostly small skin biopsies.
>
> Our acid and ammonia solutions are as follows:
>
> 1% acid alcohol
> 2000 ml of 70% EtOH
> 5 ml of HCl (hydrochloric acid)
>
> 1% Ammonia water
> 1980 cc tap water
> 40 ml of ammonium hydroxide
>
> We have tried longer xylene pre-treatment with more
> agitation, and wiping off alcohol drops before removing
> the bin from the last alcohol, as well as various staining
> adjustments. We process most of our tissue on a short 3
> hour cycle however believe this problem has persisted even
> on overnight-processed tissue.
> I would very much appreciate any advise any of you can
> give me on this problem. Thanks very much and have a
> happy holiday! Alice Neumann M.D. Precision Pathology PC,
> Arvada CO alineumann@aol.com 303-432-7855
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----------------------------------------------------------------------
Date: 24 Dec 2002 09:32:44 -0600
From: "Dawson, Glen"
Subject: Christmas Eve Question
Just out of curiosity, does anyone out there in histo-land still get
Christmas Eve off as a holiday? We got it off up until 5 years ago when we
became a reference lab. Now, all of our outside clients feel obliged to cut
their staff loose for Christmas Eve and hammer my lab with all of their work
with no feelings of guilt that I am working on a skeleton crew as well. I
was lucky enough to put in an 11 hour day yesterday and have no idea when
I'll get out of here today. I think that the Christmas spirit is something
that reference lab histotechs can only hear about because we have no time to
enjoy it ourselves. Ahhh, Christmas venting feels so good.
Bah Humbug,
Glen Dawson BS, HT & QIHC (ASCP)
Lead IHC Technologist
Milwaukee, WI
----------------------------------------------------------------------
Date: 24 Dec 2002 09:44:02 -0600
From: HACKERLAB@aol.com
Subject: Happy Holidays
Very Best Wishes for happy holidays, merry Christmas and a happy new year to
everyone out there in histoland from all of us at hacker instruments &
industries inc.
may this time be peaceful and bright!
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Very Best Wishes for happy holidays, merry
Christmas and a happy new year to everyone out there in histoland from all of
us at hacker instruments & industries inc.
may this time be peaceful and bright!
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----------------------------------------------------------------------
Date: 24 Dec 2002 10:06:29 -0600
From: Kim Merriam
Subject: [Fwd: Variability of histology staining-request for input]
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Date: Tue, 24 Dec 2002 08:42:32 -0700
From: "Mack, Jacquie"
Subject: RE: Variability of histology staining-request for input
To: 'Kim Merriam'
Message-id: <30C050525B881C4AAFF41E6D16543E6801762E99@mail3.mail.cls.ab.ca>
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I suspect that your alcohol times both going to water and coming back up to
xylene are too short. Try increasing them to at least a minute or two each
with agitation.
Best of luck
Jacquie
- -----Original Message-----
From: Kim Merriam [mailto:kmerriam@tktx.com]
Sent: December 24, 2002 7:53 AM
To: AliNeumann@aol.com
Cc: histonet@pathology.swmed.edu
Subject: Re: Variability of histology staining-request for input
I would try longer rinses in water after the hematoxylin,
acid alcohol and ammonia water (running water would probably
be best). Also I would go longer in the 95% alcohols that
are after the eosin.
Kim Merriam
TKT
Cambridge, MA
AliNeumann@aol.com wrote:
> Hi everyone, I am the director of a new small outpatient
> laboratory, and we have had a very persistent and
> recalcitrant problem with variability of staining (some
> sections light, others dark, especially on eosin but to a
> lesser degree hematoxylin). We are soaking slides in
> xylene for 3 minutes in each of 3 bins with agitation,
> then staining them on a Varistain 24-4 with a regressive
> stain. Our staining protocols have been:
>
> 1. 100% Alcohol 20 seconds
> 2. 100% Alcohol 10 seconds
> 3. 95% Alcohol 10 seconds
> 4. Tap Water 10 seconds
> 5. Hematoxylin 7 minutes
> (Protocol Harris mercury free non-acidified type)
> 6. Tap Water 20 seconds
> 7. Acid Alcohol 2 seconds
> 8. Tap Water 20 seconds
> 9. Ammonia Water 2 minutes
> 10. Tap Water 10 seconds
> 11. 95% Alcohol 10 seconds
> 12. Eosin 15 seconds
> 13. 95% Alcohol 10 seconds
> 14. 95% Alcohol 10 seconds
> 15. 100% Alcohol 20 seconds
> 16. 100% Alcohol 20 seconds
>
>
> 1. 100% Ethanol 40 seconds
> 2. 100% Ethanol 40 seconds
> 3. 95% Ethanol 40 seconds
> 4. Tap water 60 seconds
> 5. Hematoxylin 10 minutes
> ( Protocol Harris mercury free non-acidified type)
> 6. Tap water (sink) 60 seconds
> 7. Acid Alcohol 2 seconds
> 8. Tap water (sink) 60 seconds
> 9. Ammonia water 2.5 minutes
> 10. Tap water 5 minutes
> 11. 95% EtOH 1 minute
> 12. Eosin 10 seconds
> 13. 95% EtOH 10 seconds
> 14. 95% EtOH 10 seconds
> 15. 100% EtOH 40 seconds
> 16. 100% EtOH 40 seconds
>
> We have had the same problem with both protocols, and
> process mostly small skin biopsies.
>
> Our acid and ammonia solutions are as follows:
>
> 1% acid alcohol
> 2000 ml of 70% EtOH
> 5 ml of HCl (hydrochloric acid)
>
> 1% Ammonia water
> 1980 cc tap water
> 40 ml of ammonium hydroxide
>
> We have tried longer xylene pre-treatment with more
> agitation, and wiping off alcohol drops before removing
> the bin from the last alcohol, as well as various staining
> adjustments. We process most of our tissue on a short 3
> hour cycle however believe this problem has persisted even
> on overnight-processed tissue.
> I would very much appreciate any advise any of you can
> give me on this problem. Thanks very much and have a
> happy holiday! Alice Neumann M.D. Precision Pathology PC,
> Arvada CO alineumann@aol.com 303-432-7855
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----------------------------------------------------------------------
Date: 24 Dec 2002 10:09:47 -0600
From: "Morken, Tim"
Subject: Half day on Chistmas eve.RE: Christmas Eve Question
We get a half day off on Christmas eve, but only due to a Presidential
directive.
Tim Morken
CDC, Atlanta
- -----Original Message-----
From: Dawson, Glen [mailto:GDawson@Milw.Dynacare.com]
Sent: Tuesday, December 24, 2002 10:21 AM
To: histonet@pathology.swmed.edu
Subject: Christmas Eve Question
Just out of curiosity, does anyone out there in histo-land still get
Christmas Eve off as a holiday? We got it off up until 5 years ago when we
became a reference lab. Now, all of our outside clients feel obliged to cut
their staff loose for Christmas Eve and hammer my lab with all of their work
with no feelings of guilt that I am working on a skeleton crew as well. I
was lucky enough to put in an 11 hour day yesterday and have no idea when
I'll get out of here today. I think that the Christmas spirit is something
that reference lab histotechs can only hear about because we have no time to
enjoy it ourselves. Ahhh, Christmas venting feels so good.
Bah Humbug,
Glen Dawson BS, HT & QIHC (ASCP)
Lead IHC Technologist
Milwaukee, WI
----------------------------------------------------------------------
Date: 24 Dec 2002 10:25:03 -0600
From: Kari Bradshaw
Subject: RE: Christmas Eve Question
Christmas Eve is just like any other day here, except more people than usual
are on vacation. Actually don't feel too bad...our lab runs with a
pathologists and gross assistant on all Holidays that fall on a Monday;
President's Day, Memorial Day, and Labor Day. Now that's tough!
- -----Original Message-----
From: Dawson, Glen [mailto:GDawson@Milw.Dynacare.com]
Sent: Tuesday, December 24, 2002 7:21 AM
To: histonet@pathology.swmed.edu
Subject: Christmas Eve Question
Just out of curiosity, does anyone out there in histo-land still get
Christmas Eve off as a holiday? We got it off up until 5 years ago when we
became a reference lab. Now, all of our outside clients feel obliged to cut
their staff loose for Christmas Eve and hammer my lab with all of their work
with no feelings of guilt that I am working on a skeleton crew as well. I
was lucky enough to put in an 11 hour day yesterday and have no idea when
I'll get out of here today. I think that the Christmas spirit is something
that reference lab histotechs can only hear about because we have no time to
enjoy it ourselves. Ahhh, Christmas venting feels so good.
Bah Humbug,
Glen Dawson BS, HT & QIHC (ASCP)
Lead IHC Technologist
Milwaukee, WI
----------------------------------------------------------------------
Date: 24 Dec 2002 10:25:21 -0600
From: "Charles.Embrey"
Subject: FW: The Reason for the Season
- -----Original Message-----
From: Charles.Embrey
Sent: Tuesday, December 24, 2002 8:24 AM
To: 'sloanaroni@netscape.net'
Subject: RE: The Reason for the Season
A little cranky are we? Sorry but when I logged onto histonet I don't
remember anywhere in the welcome e-mail that I was to strictly limit my
correspondence to histology only. We are a community of people not
histology robots. Use your delete key if the subject line doesn't interest
you and quit your whining. smile a little and enjoy life.
"You will be visited be three spirits, the first when the clock strikes
one......."
Charles R. Embrey Jr. PA(AAPA), HT(ASCP)
Histology Manager
Carle Clinic, Urbana Illinois
- -----Original Message-----
From: sloanaroni@netscape.net [mailto:sloanaroni@netscape.net]
Sent: Monday, December 23, 2002 3:12 PM
To: CTague@ahs.llumc.edu; lesley@vancouverbc.net;
bill501@mindspring.com; histonet@pathology.swmed.edu
Subject: RE: The Reason for the Season
How have we gone from Histology to Theology? This is not what this list
server is for. I don't know about you guys but I don't have time for
religious philisophical banter when I check my email-
"Tague, Curtis" wrote:
>Actually, the reason for the season is the BIRTH (not rebirth) of the SON
(not sun).
>
>God bless you, my friend.
>
> -----Original Message-----
>From: Lesley Weston [mailto:lesley@vancouverbc.net]
>Sent: Sunday, December 22, 2002 09:24
>To: Bill Blank; histonet@pathology.swmed.edu
>Subject: Re: The Reason for the Season
>
>on 22/12/2002 8:43 AM, Bill Blank at bill501@mindspring.com wrote:
>
>>> I agree my sister. JESUS IS THE REASON FOR THE SEASON NOT JUST PRESENTS
OR
>>> SANTA CLAUS
>>
>> Actually the reason for the season is the rebirth of the Sun on the
>> Winter Solstice.
>>
>
>Mostly, the reason for the season is to cheer us all up because we've all
>got SAD. This has tremendous relevance to histology.
>
>Lesley Weston.
>
>
>
>
>
>
- --
"Great spirits have often encountered violent opposition from mediocre
minds"
A. Einstein
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----------------------------------------------------------------------
Date: 24 Dec 2002 10:37:42 -0600
From: JHoffpa464@aol.com
Subject: Re: Half day on Chistmas eve.RE: Christmas Eve Question
thats probly the only good thing the pres has done.
----------------------------------------------------------------------
Date: 24 Dec 2002 10:40:59 -0600
From: "Steve Machin UK"
Subject: Re: Christmas Eve Question
I am in an English NHS lab and we don't get Christmas eve off.
It's 4:30pm now; all the other staff left shortly after lunch so I
am just "minding the ship&#untiltill 5:15.
The place is very quiet although I understand that our out-patient
clinics are likely to run until 6pm.
So I will get two days off and then we will be back on Friday for
work as usual.
--- "Dawson, Glen" wrote: > Just out of
curiosity, does anyone out there in histo-land still
> get
> Christmas Eve off as a holiday? We got it off up until 5 years ago
> when we
> became a reference lab. Now, all of our outside clients feel
> obliged to cut
> their staff loose for Christmas Eve and hammer my lab with all of
> their work
> with no feelings of guilt that I am working on a skeleton crew as
> well. I
> was lucky enough to put in an 11 hour day yesterday and have no
> idea when
> I'll get out of here today. I think that the Christmas spirit is
> something
> that reference lab histotechs can only hear about because we have
> no time to
> enjoy it ourselves. Ahhh, Christmas venting feels so good.
>
> Bah Humbug,
>
> Glen Dawson BS, HT & QIHC (ASCP)
> Lead IHC Technologist
> Milwaukee, WI
>
__________________________________________________
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----------------------------------------------------------------------
Date: 24 Dec 2002 10:47:24 -0600
From: "Steve Machin UK"
Subject: Merry Christmas?
Merry Christmas to everyone.
Lets hope its not the last one, which it could be if there is a big
war in the Gulf.
Steve Machin UK
__________________________________________________
Do You Yahoo!?
Everything you'll ever need on one web page
from News and Sport to Email and Music Charts
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----------------------------------------------------------------------
Date: 24 Dec 2002 10:50:36 -0600
From: Instrumedics
Subject: Re: Daily Digest
To all our best wishes for a very good New Year!
Bernice
schiller@instrumedics.com
----------------------------------------------------------------------
Date: 24 Dec 2002 10:59:22 -0600
From: "Weems, Joyce"
Subject: RE: Christmas Eve Question
We're here, a couple of staff short. Yesterday was a record workload day for
us. It must be worse for the patients who we are working on - as we have
some very seriously ill folk here.
We have a lighter surgery day, but nevertheless full. We'll be back on
Thurs.
Blessings to all. j
Joyce Weems
Pathology Manager
Saint Joseph's Hospital of Atlanta
404-851-7376
404-851-7831 - fax
-----Original Message-----
From: Dawson, Glen [mailto:GDawson@Milw.Dynacare.com]
Sent: Tuesday, December 24, 2002 10:21 AM
To: histonet@pathology.swmed.edu
Subject: Christmas Eve Question
Just out of curiosity, does anyone out there in histo-land still get
Christmas Eve off as a holiday? We got it off up until 5 years ago when we
became a reference lab. Now, all of our outside clients feel obliged to cut
their staff loose for Christmas Eve and hammer my lab with all of their work
with no feelings of guilt that I am working on a skeleton crew as well. I
was lucky enough to put in an 11 hour day yesterday and have no idea when
I'll get out of here today. I think that the Christmas spirit is something
that reference lab histotechs can only hear about because we have no time to
enjoy it ourselves. Ahhh, Christmas venting feels so good.
Bah Humbug,
Glen Dawson BS, HT & QIHC (ASCP)
Lead IHC Technologist
Milwaukee, WI
----------------------------------------------------------------------
Date: 24 Dec 2002 11:15:44 -0600
From: Kemp Watson
Subject: RE: The Reason for the Season
"WHAT ARE THE RULES?
You may post any questions you wish pertaining to histology, pathology,
in-situ hybridization, immunohistochemistry etc. Equipment and reagent
evaluations, laboratory management issues, government regulations, and job
opportunities are all appropriate topics. The University asks that we
restrict
the use of its hardware and software to business purposes only..."
That was from my subscription. The reply from Curtis below may have been a
bit terse indeed, but he's not wrong.
I celebrate Christmas, but I'd hate to see the response from many on this
list when it was deluged with Happy Hanukkah/Chanukah, Happy Diwali, Happy
Ramadan, Happy Winter Solstice, Happy Eid al-Adha, etc. Non-Christians
generally have a lot more awareness of religious diversity, it seems, at
least regarding mailing list protocol.
Happy New Year doesn't work, either...
I'm not meaning to knock people's good wishes, mind you - I know they are
well intended. Perhaps we could keep the good wishes to the first 3-4
postings?
Kemp Watson
> -----Original Message-----
> From: Charles.Embrey [mailto:Charles.Embrey@carle.com]
> Sent: December 24, 2002 11:10 AM
> To: 'histonet@pathology.swmed.edu'
> Subject: FW: The Reason for the Season
>
>
>
>
> -----Original Message-----
> From: Charles.Embrey
> Sent: Tuesday, December 24, 2002 8:24 AM
> To: 'sloanaroni@netscape.net'
> Subject: RE: The Reason for the Season
>
>
> A little cranky are we? Sorry but when I logged onto histonet I don't
> remember anywhere in the welcome e-mail that I was to strictly limit my
> correspondence to histology only. We are a community of people not
> histology robots. Use your delete key if the subject line
> doesn't interest
> you and quit your whining. smile a little and enjoy life.
> "You will be visited be three spirits, the first when the clock strikes
> one......."
> Charles R. Embrey Jr. PA(AAPA), HT(ASCP)
> Histology Manager
> Carle Clinic, Urbana Illinois
>
> -----Original Message-----
> From: sloanaroni@netscape.net [mailto:sloanaroni@netscape.net]
> Sent: Monday, December 23, 2002 3:12 PM
> To: CTague@ahs.llumc.edu; lesley@vancouverbc.net;
> bill501@mindspring.com; histonet@pathology.swmed.edu
> Subject: RE: The Reason for the Season
>
>
> How have we gone from Histology to Theology? This is not what this list
> server is for. I don't know about you guys but I don't have time for
> religious philisophical banter when I check my email-
>
>
>
> "Tague, Curtis" wrote:
>
> >Actually, the reason for the season is the BIRTH (not rebirth) of the SON
> (not sun).
> >
> >God bless you, my friend.
> >
> > -----Original Message-----
> >From: Lesley Weston [mailto:lesley@vancouverbc.net]
> >Sent: Sunday, December 22, 2002 09:24
> >To: Bill Blank; histonet@pathology.swmed.edu
> >Subject: Re: The Reason for the Season
> >
> >on 22/12/2002 8:43 AM, Bill Blank at bill501@mindspring.com wrote:
> >
> >>> I agree my sister. JESUS IS THE REASON FOR THE SEASON NOT
> JUST PRESENTS
> OR
> >>> SANTA CLAUS
> >>
> >> Actually the reason for the season is the rebirth of the Sun on the
> >> Winter Solstice.
> >>
> >
> >Mostly, the reason for the season is to cheer us all up because we've all
> >got SAD. This has tremendous relevance to histology.
> >
> >Lesley Weston.
> >
> >
> >
> >
> >
> >
>
>
> --
> "Great spirits have often encountered violent opposition from mediocre
> minds"
> A. Einstein
>
>
> __________________________________________________________________
> The NEW Netscape 7.0 browser is now available. Upgrade now!
> http://channels.netscape.com/ns/browsers/download.jsp
>
> Get your own FREE, personal Netscape Mail account today at
> http://webmail.netscape.com/
>
----------------------------------------------------------------------
Date: 24 Dec 2002 11:51:01 -0600
From: Bruce Gapinski
Subject: RE: Christmas Eve Question
Same here. I'm the supervisor and I sent everyone home by 9:00 AM. They
went... kicking and screaming (You think I joke? No, I have the best
staff)I'm doing glassware and waiting for the IHC to come out and I'm GONE.
One World is Enough for All of Us!
Bruce (I am not a rep) Gapinski HT(ASCP)
- -----Original Message-----
From: Weems, Joyce [mailto:JWEEMS@sjha.org]
Sent: Tuesday, December 24, 2002 8:48 AM
To: 'Dawson, Glen'; histonet@pathology.swmed.edu
Subject: RE: Christmas Eve Question
We're here, a couple of staff short. Yesterday was a record workload day for
us. It must be worse for the patients who we are working on - as we have
some very seriously ill folk here.
We have a lighter surgery day, but nevertheless full. We'll be back on
Thurs.
Blessings to all. j
Joyce Weems
Pathology Manager
Saint Joseph's Hospital of Atlanta
404-851-7376
404-851-7831 - fax
-----Original Message-----
From: Dawson, Glen [mailto:GDawson@Milw.Dynacare.com]
Sent: Tuesday, December 24, 2002 10:21 AM
To: histonet@pathology.swmed.edu
Subject: Christmas Eve Question
Just out of curiosity, does anyone out there in histo-land still get
Christmas Eve off as a holiday? We got it off up until 5 years ago when we
became a reference lab. Now, all of our outside clients feel obliged to cut
their staff loose for Christmas Eve and hammer my lab with all of their work
with no feelings of guilt that I am working on a skeleton crew as well. I
was lucky enough to put in an 11 hour day yesterday and have no idea when
I'll get out of here today. I think that the Christmas spirit is something
that reference lab histotechs can only hear about because we have no time to
enjoy it ourselves. Ahhh, Christmas venting feels so good.
Bah Humbug,
Glen Dawson BS, HT & QIHC (ASCP)
Lead IHC Technologist
Milwaukee, WI
----------------------------------------------------------------------
Date: 24 Dec 2002 11:58:23 -0600
From: Valleygal@aol.com
Subject: RE: The Reason for the Season
You just have to love the personalities on this listserver!
This is indeed a diverse group of folks - whatever your belief, be it
religious, commercial, astrological or even if you are Scrooge...this is your
time to celebrate and enjoy. So...let the celebration and enjoyment commence!
Andi Grantham
**********************************************************************
=^..^=
******************* NOTE *******************
There may be important message content
contained in the following MIME Information.
********************************************
- ------------------ MIME Information follows ------------------
- --Boundary_(ID_cHVE4uECYGEQ+6VIxduuwQ)
Content-type: text/plain; charset=US-ASCII
Content-transfer-encoding: 7BIT
<<<<<< See above "Message Body" >>>>>>
- --Boundary_(ID_cHVE4uECYGEQ+6VIxduuwQ)
Content-type: text/html; charset=US-ASCII
Content-transfer-encoding: 7BIT
You just have to love the personalities on this listserver!
This is indeed a diverse group of folks - whatever your belief, be it
religious, commercial, astrological or even if you are Scrooge...this is your
time to celebrate and enjoy. So...let the celebration and enjoyment
commence!
Andi Grantham
**********************************************************************
=^..^=
- --Boundary_(ID_cHVE4uECYGEQ+6VIxduuwQ)--
----------------------------------------------------------------------
Date: 24 Dec 2002 12:01:37 -0600
From: Cynthia Favara
Subject: RE: Individual characteristics
Short, sweet and WISE!!!
Peace to ALL
Cynthia Favara
- -----Original Message-----
From: RUSS ALLISON [mailto:Allison@Cardiff.ac.uk]
Sent: Tuesday, December 24, 2002 1:51 AM
To: histonet@pathology.swmed.edu
Subject: Individual characteristics
Tolerance is a virtue
Russ Allison,
Dental School
Cardiff
Wales
----------------------------------------------------------------------
Date: 24 Dec 2002 13:22:58 -0600
From: "Dawson, Glen"
Subject: RE: Christmas Eve
All,
Misery truly loves company. Thanx to all who let me know that I am not
alone in my boat.
Cheers,
Glen Dawson
----------------------------------------------------------------------
Date: 24 Dec 2002 17:13:30 -0600
From: Carlos Defeo
Subject: Re:variability on staining
Dear Ali:
Can you tell the formula of the eosin solution you are using? For the
changes in ethanol 95 I suppose you are employing an alcoholic solution.
I recommend an aquous solution of eosin Y 0,75-1%
and ommit then the wash in water,passing trough graded alcoholic baths. On
this way you will have to change the first 95 ethanol dehydrating bath
frequently but overdifferenciation of eosin will be avoided.
Carlos,Montevideo,Uruguay.
Here are the messages received yesterday!
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