|From:||Kim Saunders |
I'm trying to work up the DBA44 (Dako) on the Ventana ES IHC Stainer. I am currently trying the recommended 1:25 dilution for 32 minutes. I've tried our NordicWare pressure cooker Antigen Retrieval method (20 in the microwave, cool down for 20 minutes), and Protease 1 for 4 & 8 minutes, and Protease 2 for 8, 12, 16, & 20 minutes. I have a bone marrow bx that the pathologist is sure should be positive based on the flow results. Since my ES uses heat, could the heat be an issue? Any help would be greatly appreciated.
St. Joseph Hospital-Tucson, AZ