Analysis of sirius red with polarized light
In the process of analysis of our picro sirius red stained sections we ran
into a discussion on the microscope prefs. We could not come to a conclusion
as to how much light must be given. We use 2 polarization filters in crossed
position. Some people want to give minimal light in order to prevent=20
overestimation of collagen content, total background is black. Others say to
give full light, but than the background becomes somewhat blueish, which
cannot be turned to black by adjusting the filters.
Does anyone have experience with different lighting on sirius sections? Or is
anyone aware of literature in which this problem is discussed.
Thanks in advance,
Dept. of Experimental Cardiology
Thoraxcentre, Erasmus MC Rotterdam
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