poly-lysine coated slides
Dear all:
Is anybody collecting frozen tissue specimens on poly-lys coated glass
slides? In order to save cost, we manually coat glass slides with
poly-L-Lysine (from Sigma), and keep them refrigerated at +4oC for a
max time of 2-3 weeks before deeming them unusable (presumably due to
poly-Lys breakdown). As a large part of our target tissue is neural,
we prefer to use this method of coating slides.
Does anybody have any clues if coated slides can last for longer
periods without losing their Lys-charge? I'm finding that too many
slides are being wasted due to the time barrier and am not particularly
sure it's reasonable (however, don't want my analysis to suffer due to
tissue/slide problems!).
.:---------------------------:.
Marcus Andrews, PhD.
Department of Physiology,
Faculty of Medicine,
University of Toronto,
1 King's College Circle,
Toronto, Ontario,
M5S 1A8
':---------------------------:'
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