brain question

From:"Anacko, Nancy L."

A research tech is having a problem.....she is doing stains on fresh frozen
brain that she recieves from a brain bank. The problem is ice-artifact on
her sections/stains. She told me that she recieves the brain at -70 and then
cuts them on a cryostat at -20. Any suggestions....I only work with fixed
brain in parrafin.
Thanks Nancy

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