Re: patchy pattern
2 things come to mind: one is that the slides are drying out while you are
staining them. This will give an uneven pattern that will also be present on
the edges of the tissue. Try placing a coverslip(glass or plastic) over the
section when staining. Make sure that you use a new one for each step of the
incubation, otherwise you'll end up with contamination.
The second reason could be that the sections have not dried cmpletely before
deparrafinization, and that small spaces are present between the section and
the slide. make sure that the sections dry completely, by standing them in
an upright position (overnight is best) and then heat them in a 60deg oven
to melt the wax before deparaffination.
Bone Research Unit
Tel & fax +27 11 717 2298
"Time flies like an arrow, fruit flies like a banana"
>From: Carlos Defeo
>Subject: patchy pattern
>Date: Mon, 09 Dec 2002 02:06:12 -0300
> Hi all:
>I would like if you could give me any good explanation to the patchy
>that gives me distinct marks of broad spectrum citokeratin and also
>cocktails of it on some cases on paraffin sections.Sections are mount on
>I can#180#t give some usefull data like condition of fixation because I receive
>the material as a paraffin block.In all cases I practiced antigen retrieval
>and of course this pattern of expression does not appear always,only in
>some kind of materials. Give me please some ideas. Thanks to all.
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