Hi Carlos,
2 things come to mind: one is that the slides are drying out while you are 
staining them. This will give an uneven pattern that will also be present on 
the edges of the tissue.  Try placing a coverslip(glass or plastic) over the 
section when staining. Make sure that you use a new one for each step of the 
incubation, otherwise you'll end up with contamination.

The second reason could be that the sections have not dried cmpletely before 
deparrafinization, and that small spaces are present between the section and 
the slide. make sure that the sections dry completely, by standing them in 
an upright position (overnight is best) and then heat them in a 60deg oven 
to melt the wax before deparaffination.

Good luck.
Louise Renton
Bone Research Unit
MRC
Johannesburg
South Africa
Tel & fax +27 11 717 2298
"Time flies like an arrow, fruit flies like a banana"





>From: Carlos Defeo 
>To: histonet@pathology.swmed.edu
>Subject: patchy pattern
>Date: Mon, 09 Dec 2002 02:06:12 -0300
>
>  Hi all:
>I would like if you could give me any good explanation to the patchy 
>pattern
>that gives me distinct marks of broad spectrum citokeratin and also
>cocktails of it on some cases on paraffin sections.Sections are  mount on
>silanyzed slides.
>I can#180#t give some usefull data like condition of fixation because I receive 
>the material as a paraffin block.In all cases I practiced antigen retrieval 
>and  of  course this pattern of expression does not appear  always,only in 
>some kind of materials. Give me please some ideas. Thanks to all.


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