Re: Storage of frozen sections 2
We have done about the same thing as you described.
Cells (attached to glass slide) or tissue sections were immersed in=20
chloroform for 5 min (defatting - we are working with atherosclerotic=20
material!!) and air-dried. Next, slides were fixed using home-made 4% PFA
for 5 minutes and washed (3x5 min) with PBS. After a brief rinse with=20
distilled water (2 min) slides were and air-dried under a fan. Stored at
-80°C (as described in previous mail) until use.
This procedure worked fine for both IHC and ISH. Morphology was good.
Chris van der Loos
Academic Medical Center H0-120
Dept. of Cardiovascular Pathology
NL-1105 AZ Amsterdam
Gayle Callis wrote:
>Date: 10 Dec 2002 14:16:06 -0600
>From: Gayle Callis
>Subject: Re: Storage of frozen sections
>A technic put out by Pharmingen on formalin or PFA fixed cells, probably
>works for tissue sections - fix in formalin, rinse with PBS X 2 changes,
>immerse slides in 1% BSA (Jackson has protease/immunglobulin free BSA) for
>10 min, remove slides but DO NOT RINSE, just air dry, and store at -80C.
>I assume BSA coats section, providing some "protein carrier barrier" from
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