Re: Storage of frozen sections

From:Gayle Callis

A technic put out by Pharmingen on formalin or PFA fixed cells, probably
works for tissue sections - fix in formalin, rinse with PBS X 2 changes,
immerse slides in 1% BSA (Jackson has protease/immunglobulin free BSA) for
10  min, remove slides but DO NOT RINSE, just air dry, and store at -80C.
I assume BSA coats section, providing some "protein carrier barrier" from
freezer atmosphere.
Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
S. 19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367 (lab with voice mail)
406 994-4303 (FAX)


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