Re: Storage of frozen sections
The foil-seal strategy wouldn't work for our purposes because we have to
select slides from individual boxes, keeping frozen of course, to constitute
a cohort of slides for a study. But I've also heard of placing the slides
into formalin (for fixation at time of experiment) in the freezer, i.e.
without thawing them first--might that also accomplish the same end? Thanks
for any advice! Susan
----- Original Message -----
From: "Patsy Ruegg"
Sent: Tuesday, December 10, 2002 12:56 PM
Subject: RE: Storage of frozen sections
> I cut frozen sections and let them airdry at least one hour and preferible
> overnight before wrapping them individually in foil, I just take a long
> piece of foil and roll the slides one over the other to seal them, I
> put them in groups of 10 or so, so I only have to take out a group rather
> that the whole batch when ready to stain. Then I place them in a zip lock
> bag with some dessicant in the bottom. They are stored at -70 until
> To remove the slides for staining, I carry a zip lock bag or plastic food
> storage container with a sealed lid which has a good layer of dessicant in
> the bottom to the -70 and working quickly in the freezer I remove one foil
> sealed group of slides and quickly place it in the transport container,
> reseal both and let the removed slides sit sealed with dessicant at RT for
> min. of 30 minutes, usually 1 hour. Now when I remove the slides sitting
> RT they are RT and dry. They would then be ready to fix and stain.
> Patsy Ruegg, HT(ASCP)QIHC
> IHCtech, LLC
> Fitzsimmons BioScience Park
> 12635 Montview Blvd. Suite 216
> Aurora, CO 80010
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> ----Original Message Follows----
> From: Gayle Callis
> To: Patsy Ruegg , firstname.lastname@example.org
> Subject: RE: Storage of frozen sections
> Date: Tue, 10 Dec 2002 10:37:20 -0700
> Patsy and Chris,
> Could you elaborate on how these sections are stored, in slide boxes (what
> kind?) sealed, zip lock baggies, etc? With dessicant in containers??? How
> long do you air dry before storage, and how long do you let them air dry
> before opening a container to prevent water condensation?? and before
> actual fixation.
> This would be helpful and I know there is additional information in
> Histonet Archives - was discussed a few years ago -
> Gayle Callis
> Research Histopathology Supervisor
> Veterinary Molecular Biology - Marsh Lab
> Montana State University - Bozeman
> S. 19th and Lincoln St
> Bozeman MT 59717-3610
> 406 994-6367 (lab with voice mail)
> 406 994-4303 (FAX)
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