Hi Rosalba

This is the method we use.


Protocol for sialinization of slides

  Mix 10ml silane within 500ml acetone (solution is stable for 8 hours - the
colour will change with time), Dip clean (acid washed), dry slides in silane
solution for 2 minutes.  Dry slides in fume hood. Wash slides with 2 changes
of water (distilled). Dry completely in an oven and cool.

Hope this helps



Zenobia Haffajee

Aiplabs

Australia

----- Original Message -----
From: "Rosalba Zumbo" 
To: "Histonet" 
Sent: Monday, November 25, 2002 3:43 PM
Subject: Silanising slides


> Hi All,
> Does anyone please have a rliable method for silanising slides. Different
> methods I have come across have varying results in regard to silver
> staining.
> Thanks
> Rosalba Zumbo
> Histology Manager
> Department of Forensic Medicine
> 50 Parramatta Rd
> Glebe NSW 2037
> PH: 02 85847842
> FAX: 02 95664573
>
>
>