Re: Kappa/Lambda IHC

From:Richard Cartun

See "Immunohistochemical Detection of Immunoglobulin Light Chain
Expression in B-Cell Non-Hodgkin Lymphomas Using Formalin-Fixed,
Paraffin-Embedded Tissues and a Heat-Induced Epitope Retrieval
Technique" by Marshall-Taylor CE, et al. in Applied Immunohistochemistry
& Molecular Morphology 10(3):258-262, 2002.

R. Cartun

>>> Cheryl Powell  12/07/02 12:41PM >>>

Hi, Botsford recently started up an immuno program and we're constantly
adding antibodies.  Kappa/Lamda has been a real pain.  We've tried
several companies and so many varations it makes my head spin- but
nothing is consistant.  What companies, dilutions, and antigen
retrievals are any of you using?  Do you have alot of background or
nonspecific staining?

Cheryl Ann Powell B.S., HTL(ASCP) 
Botsford General Hospital 

Farmington Hills, MI USA 

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