Re: Dissolving skeletal muscle from bone

From:Philip Oshel

I've used trypsin, KOH, and NaOH, each separately. I forget the % 
trypsin, but I used 1 molar KOH or NaOH. I've also used Chlorox. 
Again, I forget the %, but I think it was around 10%.


>Hi all,
>Does anyone know what solution to use to dissolve skeletal
>muscle off of bone, so that the bone remains in tact.  This
>is not for a histology study, but I thought that someone out
>there on the histonet would know how to do this!
>Thanks in advance,
>Kim Merriam
>Cambridge, MA
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison,  WI  53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)

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