Re: Cracks in Paraffin Wax Blocks
>>> Sharron Ladd 12/04/02 11:30AM >>>
Are you using metal molds? I learned in a NSH workshop, that leaving
blocks chilling too long on the cold plate (in metal molds) can cause
cracks to form in the paraffin.
>Please could anybody tell me why I am getting cracks in my paraffin
>blocks? I noticed someone had asked this question a couple of years
>but there was no reply posted. I can still cut sections from these
>and they look fine under the microscope. Not all the blocks in a
>cracked, some are nice and I've even had batches where 90% of the
>are good and only a few have cracks.
>We use Surgipath Formula R paraffin wax and I have spoken to the rep
>and he suggested a couple of things, but none have made any
>has also checked the batch of wax we were using and there are no
>problems; besides we are now onto another batch and the same thing
> I have wondered whether it is my embedding technique, but have tried
>different ways, e.g. different amounts of wax in the base mould before
>orientation, different lengths of time on the refrigeration spot
>topping up and different methods of cooling, ie. using the coldplate
>-20 freezer. I am also careful not to move the tissue once the wax
>to solidify. The wax is melted at 60 degrees celsius in the processor
>the embedding centre is also set at 60.
>I would be very grateful if anyone has anyone got any suggestions?
>Thanking you in advance.
>Is someone spraying the blocks with freeze spray to get them cold
faster? That will make cracks.
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