Re: Cox1 & 2 Abs

From:Greg Dobbin

Hi Lin,
I got COX-2 to work in rat lungs using COX-2 (murine) Polyclonal 
Ab from Cayman Chemical (Cat. # 160126). At that time I was 
using citrate buffer retreival in steam for 20 mins. and overnight 
incubation at 4 C. My detection system was the LSAB2 kit from 

Subsequently, in trying to get canine tissues to stain, I have 
modified to no retreival and 60-90 mins at 37 C incubation. I am still 
getting a good signal (I think!) but I ahve some annoying nonsp. rx in 
my deletion controls that I am having trouble finding the source of. 
But I think that is an issue of species, not protocol per se.
Cox-2 does seem to be a "finicky" one to get to work!
Good luck.

Date sent:      	Tue, 10 Dec 2002 19:56:00 -0500 (EST)
Subject:        	Cox1 & 2 Abs

> Hello,
> I would greatly appreciate a source for Cox1 & 2 Abs and a working protocol 
> for
> FFPE rodent tissue(brain).
> Thanks,
> Lin

Greg Dobbin
Pathology Lab
Atlantic Veterinary College, U.P.E.I.
550 University Ave.
Charlottetown, P.E.I.
Canada,  C1A 4P3
Phone: (902)566-0744
Fax: (902)566-0851
"A farmer is a person outstanding in their field."

<< Previous Message | Next Message >>