Re: Cox1 & 2 Abs
I got COX-2 to work in rat lungs using COX-2 (murine) Polyclonal
Ab from Cayman Chemical (Cat. # 160126). At that time I was
using citrate buffer retreival in steam for 20 mins. and overnight
incubation at 4 C. My detection system was the LSAB2 kit from
Subsequently, in trying to get canine tissues to stain, I have
modified to no retreival and 60-90 mins at 37 C incubation. I am still
getting a good signal (I think!) but I ahve some annoying nonsp. rx in
my deletion controls that I am having trouble finding the source of.
But I think that is an issue of species, not protocol per se.
Cox-2 does seem to be a "finicky" one to get to work!
Date sent: Tue, 10 Dec 2002 19:56:00 -0500 (EST)
Subject: Cox1 & 2 Abs
> I would greatly appreciate a source for Cox1 & 2 Abs and a working protocol
> FFPE rodent tissue(brain).
Atlantic Veterinary College, U.P.E.I.
550 University Ave.
Canada, C1A 4P3
"A farmer is a person outstanding in their field."
<< Previous Message | Next Message >>