RE: poly-lysine coated slides

From:"Philopena, Jennifer"

Poly-L-lysine comes in different molecular weights.  Which do you use?

-----Original Message-----
From: Browning Deb [mailto:browning@HHSC.CA]
Sent: Thursday, December 05, 2002 7:14 AM
To:; 'Marcus Andrews'
Subject: RE: poly-lysine coated slides

We are also using the Sigma poly-l-lysine routinely in our lab.  I know from
experience that the coated slides will last much longer than 3 weeks, at
room temperature.  We store control slides precut on poly-l slides,
sometimes for more than a year if the antibody in question is obscure.  We
use the same poly-l slides for our frozen muscle immuno staining, and other
than the "static jumping" of the section onto the coated slide, we have
never experienced the tissue lifting off.  Of course, now that I have said
Our ffpe neuro tissue stays on the slide as well.

	From:  Marcus Andrews[]
	Sent:  Wednesday, December 04, 2002 4:30 PM
	Subject:  poly-lysine coated slides

	Dear all:

	Is anybody collecting frozen tissue specimens on poly-lys coated
	slides?  In order to save cost, we manually coat glass slides with 
	poly-L-Lysine (from Sigma), and keep them refrigerated at +4oC for a

	max time of 2-3 weeks before deeming them unusable (presumably due
	poly-Lys breakdown).  As a large part of our target tissue is
	we prefer to use this method of coating slides.

	Does anybody have any clues if coated slides can last for longer 
	periods without losing their Lys-charge?  I'm finding that too many 
	slides are being wasted due to the time barrier and am not
	sure it's reasonable (however, don't want my analysis to suffer due
	tissue/slide problems!).

	   Marcus Andrews, PhD.
	   Department of Physiology,
	   Faculty of Medicine,
	   University of Toronto,
	   1 King's College Circle,
	   Toronto, Ontario,
	   M5S 1A8

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