RE: poly-lysine coated slides
We are also using the Sigma poly-l-lysine routinely in our lab. I know from
experience that the coated slides will last much longer than 3 weeks, at
room temperature. We store control slides precut on poly-l slides,
sometimes for more than a year if the antibody in question is obscure. We
use the same poly-l slides for our frozen muscle immuno staining, and other
than the "static jumping" of the section onto the coated slide, we have
never experienced the tissue lifting off. Of course, now that I have said
Our ffpe neuro tissue stays on the slide as well.
From: Marcus Andrews[SMTP:email@example.com]
Sent: Wednesday, December 04, 2002 4:30 PM
Subject: poly-lysine coated slides
Is anybody collecting frozen tissue specimens on poly-lys coated
slides? In order to save cost, we manually coat glass slides with
poly-L-Lysine (from Sigma), and keep them refrigerated at +4oC for a
max time of 2-3 weeks before deeming them unusable (presumably due
poly-Lys breakdown). As a large part of our target tissue is
we prefer to use this method of coating slides.
Does anybody have any clues if coated slides can last for longer
periods without losing their Lys-charge? I'm finding that too many
slides are being wasted due to the time barrier and am not
sure it's reasonable (however, don't want my analysis to suffer due
Marcus Andrews, PhD.
Department of Physiology,
Faculty of Medicine,
University of Toronto,
1 King's College Circle,
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