RE: brain question

From:Bill Blank

>I think the problem is in the initial freezing of the brains. Ice crystals
>will grow if the tissue is not frozen fast enough i.e. snap frozen in liquid

Better in an isopentane slurry in LN2. The isopentane has less of an 
insulating effect when it evaporates than LN2 alone. The best way of 
course is to use a brain blower which can give homogenous water ice.

Wm F Blank MD
Heartland Laboratory, Inc
Chaffee, MO 63740

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