I think the problem is in the initial freezing of the brains. Ice crystals
will grow if the tissue is not frozen fast enough i.e. snap frozen in liquid
nitrogen.
Rita.

-----Original Message-----
From: Anacko, Nancy L. [mailto:NAnacko@Lifespan.org]
Sent: 06 December 2002 15:37
To: 'Server, Histonet'
Subject: brain question


Hi,
A research tech is having a problem.....she is doing stains on fresh frozen
brain that she recieves from a brain bank. The problem is ice-artifact on
her sections/stains. She told me that she recieves the brain at -70 and then
cuts them on a cryostat at -20. Any suggestions....I only work with fixed
brain in parrafin.
Thanks Nancy